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Volume 1, Issue 1

International Journal of Science and Engineering Applications (IJSEA)
Volume 1, Issue 1 - November 2012

Effect of synthetic carbon substrates and cane molasses, an agro waste
on exopolysaccharide production by P. fluorescens

A. Sirajunnisa, V.Vijayagopal, T.Viruthagiri

10.7753/IJSEA0101.1010




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Abstract:

Exopolysaccharides (EPS) from P.fluorescens was produced using sucrose and sugarcane molasses as the carbon substrates at different concentrations (1-7%), at different incubation time (12, 24, 36, 48, 60 and 72 hr). The extraction was carried out using ethanol precipitation technique. The lyophilized samples were analysed for its total carbohydrates content. The predominant sugar was found to be glucose by TLC. The functional groups were identified using FT-IR spectroscopy. Maximum production was given by the medium containing 5% sugarcane molasses and was found to be 2843 mg/l at 48 hr after which the production decreased. The EPS production using sugarcane molasses gave comparatively a higher yield than sucrose, which could be commercialized for a cost effective production of this viscous to plastic polymers.

Keywords: Pseudomonas fluorescens, sugarcane molasses, exopolysaccharides, FT-IR spectroscopy, biosurfactants.

References:

[1] Filomena, F., Alves, V.D., and Maria Reis, A.M. 2011. Advances in bacterial Exopolysaccharides: from production to biotechnological applications. Trends in Biotechnology, 29 (8), 388-398.

[2] Bhaskar, P.V., and Bhosle, N.B. 2006. Baterial extracellular polymeric substance carrier of heavy metals in the marine food-chain. Environ Int. 32 (2), 191-198.

[3] Hinsa, S.M., and O’Toole, G.A. 2006. Biofilm formation by Pseudomonas fluorescens WCS365: a role for LapD. Microbiology. 152, 1375-1383.

[4] Pamp, S.J., Gjermansen, M., and Tolker-Nielsen, T. The biofilm Matrix: A Stricky Framework.

[5] Bryan, B.A., Linhardt, R. J., and Daniels, L. 1986. Variation in composition and yield of exopolysaccharides produced by Klebsiella sp. strain K32 and Acenitobacter calcoaceticus BD4. Appl. Environ. Microbiol. 51(6), 1304-1308.

[6] Surekha, K., Satpute, Ibrahim, Banat, M., Prashant, K., Dhakephalkar, Banpurkar, A.G., Balu, Chopade, A. 2010. Biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms. Biotechnology Advances. 28, 436-450.

[7] Poli, A., Anzelmo, G., and Nicolaus, B. 2010. Bacterial Exopolysaccharides from Extreme Marine Habitats: Production, Characterization and Biological Activities. Mar. Drugs 8, 1779-1802.

[8] de Oliveira Martins, P.S., de Almeida, N.F., and Ferreira Leite, S.G, 2008. Application of a bacterial extracellular polymeric substance in heavy metal adsorption in a co-contaminated aqueous system. Brazilian Journal of Microbiology. 39, 780-786.

[9] Moppert, X., Le Costaouec, T., Ragunenes, G., Courtois, A., Simon- Colin, C., Crassous, P., Costa, B., and Guezennec, J. 2009. Investigations into the uptake of copper, iron and selenium by a highly sulphated bacterial exopolyaccharide isolated from microbial mats. Journal of Industrial Microbiology and Biotechnology. 36(4), 599-604.

[10] Kocharin, K., Rachathewe, P., Sanglier, J.J., and Prathumpai, W. 2010. Exobiopolymer production by Ophiocordyceps diterigena BCC 2073: optimization, production in bioreactor and characterization. BMC Biotechnology. 10 (51).

[11] Onbasli, D., and Aslim, A. 2008. Determination of antimicrobial activity and production of some metabolites by Pseudomonas aeruginosa B1 and B2 in sugar beet molasses. African Journal of Biotechnology. 7 (24), 4614-4619.

[12] Liu, J., Luo, J., Ye, H., Sun, Y., Lu, Z., and Zeng, X. 2010. In vitro and in vivo antioxidant activity of exopolysaccharides from endophytic bacterium Paenibacillus polymyxa EJS-3. Carbohydrate Polymers. 82, 1278-1283.

[13] Liu, C.T., Chu, F.J., Chou, C.C., and Yu, R.C. 2011. Antiproliferative and anticytotoxic effects of cell fractions and exopolysaccharides from Lactobacillus casei 01. Mutation Research.

[14] Banik, R. M., Kanari, B., and Upadhyay, S.N. 2000. Exopolysaccharide of the gellan family: Prospects and potential. World Journal of Microbiology. 16, 407-414.

[15] Williams, A.G., and Wimpenny, J.W.T. 1977. Exopolysaccharide Production by Pseudomonas NCIB~ 1264 Grown in Batch Culture. Journal of General Microbiology, 102, 13-21.

[16] Palleroni, N.J. 1984. Pseudomonadaceae - Bergey’s Manual of Systematic Bacteriology. Krieg, N. R. and Holt J.G. (editors) Baltimore: The Willliams and Wilkins Co. 141-149.

[17] Osman, S.F., Fett, W.F., Irwin, P., Brouillette, J.N., and Connor, J.V.O. 1997. The structure of the exopolysaccharides of Pseudomonas fluorescens strain H13. Carbohydrate Research. 300, 323-327.

[18] Hung, C.C., Santschi, P.H., and Gillow, J.B. 2005. Isolation and characterization of extracellular polysaccharides produced by Pseudomonas fluorescens Biovar II. Carbohydrate Polymers. 61,141–147.

[19] Celik,G.Y., Aslim, B., and Beyatil, Y. 2008. Characherization and production of the exoplysaccharide (EPC) from Pseudomonas aeruginosa GI and Pseudomonas putida G12 strains. Carbohydrate Polymers. 73, 178-182.

[20] Fett, W.F. 1993. Bacterial exopolysaccharides: Their nature, regulation and role in host-pathogen interactions. Current Topics in Botanical Research, 1, 367-390.

[21] De Vuyst, L., and Degeest, B. 1999. Heteropolysaccharides from lactic acid bacteria. FEMS Microbiology Reviews, 23, 153-177.

[22] Muthusamy, K., Gopalakrishnan, S., Kochupappy Ravi, T., and Sivachidambaram, P. 2008. Biosurfactants: Properties, Commercial production and application. Current science. 94 (6).

[23] Olbrich, H. 2006. The Molasses. Biotechnologie-Kempe GmbH Publishers. pp. 1-131.

[24] Fusconi, R., Lezi Godinno, M.J., and Segnini Bossolan, N.L., 2008. Culture and exopolysaccharides production from sugarcane molasses by Gordonia polyisoprenivorans CCT 7137, isolated from contaminated groundwater in Brazil. World Journal of Microbiology and Biotechnology. 24, 7, 937-943.

[25] Savadogo, A., Savadogo, C.W., Barro, N., Ouattara, A.S., Traore, A.S. 2004. Identification of exopolysaccharides producing lactic acid bacteria from Burkino Faso fermented milk samples. African Journal of Biotechnology. 3(3). 189-194.

[26] Dubois, M., Giles, K.A., Hamilton, J. K. Rebers, P. A. and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350-356.

[27] Moosavi-Nasab, M., Shekaripour, F. and Alipoor, M. 2008 & 2009. Use of Date Syrup as Agricultural Waste for Xanthan Production by Xanthomonas campestris. Iran Agricultural Research. 27(1-2), and 28(1).

[28] Patil, S.V., Patil, C. D., Salunke, B.K., Salunkhe, R.B., Bathe, G.A., and Patil, D.M. 2011. Studies on Characterization of Bioflocculant Exopolysaccharide of Azotobacter indicus and Its Potential for Wastewater Treatment. Appl Biochem Biotechnol., 163:463–472.

[29] Rui, L., Wei-Chang, C., Peng, W., Yan, T.W., Guang, Z.G., 2009. Extraction, characterization of Astragalus polysaccharides and its immune modulating activities in rats with gastric cancer. Carbohydrate Polymers. 78,738- 742.

[30] Ibrahim, M., Alaam, M., EI-Haes, H., Jalbout, A.F., de Leon, A. 2006. Analysis of the structure and vibrational spectra of glucose and fructose, Ecl. Quim., Sao Paulo, 31(3): 15-21.

[31] Parikh, A., and Madamwar, D. 2006. Partial characterization of extracellular polysaccharides from Cyanobacteria. Bioresource Technology 97, 1822-1827.

[32] Hung, C.C., Santschi, P.H., and Gillow, J.B. 2005. Isolation and characterization of extracellular polysaccharides produced by Pseudomonas fluorescens Biovar II. Carbohydrate Polymers, 61, 141–147.