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Volume 1, Issue 2

International Journal of Science and Engineering Applications (IJSEA)
Volume 1, Issue 2 - December 2012

Production of Tannase using Aspergillus niger by Submerged Fermentation

Mohan Kuppusamy, Viruthagiri Thangavelu, Suresh Baladhandayutham

10.7753/IJSEA0102.1010




 PDF 


Abstract:

A study on optimization of submerged fermentative production of tannase by Aspergillus niger was carried out. Aspergillus niger (MTCC 3557) was used for maximum production of Tannase enzyme and the factors affecting the production were optimized. The optimum conditions for maximum tannase enzyme production in the Submerged Fermentation (SmF) using Aspergillus niger was obtained with an initial tannic acid concentration of 3% (w/v) at 35°C, Initial pH of 5.5 and a fermentation period of 96h.The substrates Betalnut husk and Plantain flower gave a maximum tannase production of 19.3 U/ml and 17.89 U/ml respectively.

Keywords: Submerged fermentation, Tannase, Agro residue, Aspergillus niger. optimization.

References:

[1] Banerjee Rintu, Mukherjee Gargi and Patra, Krushna Chandra. May 2005, Microbial transformation of tannin-rich substrate to gallic acid through co-culture method.Bioresource Technology, vol. 96, no. 8, p. 949- 953.

[2] Hadi, T.A., Banerjee, R., Bhatacharya, B.C., 1994.Optimization of tannase biosynthesis by a newly isolated Rhizopus oryzae. Bioprocess Eng. 11, 239–243.

[3] Bradoo, S., Gupta, R., and Saxena, R. K. ., 1996 Screening of extracellular tannase producing fungi. Development of a rapid and simple plate assay, J. Gen. Appl. Microbiol., 42, 325–329.

[4] Batra, A. and Saxena, R. K., 2005, Potential tannase producers from the genera Aspergillus and Penicillium, Process Biochem., 40, 1553–1557.

[5] Murugan, K., Saravanababu, S., and Arunachalam, M., 2007, Screening of tannin acyl hydrolase (E.C.3.1.1.20) producing tannery effluent fungal isolates using simple agar plate and SmF process, Bioresour. Technol., 98, 946–949.

[6] Paranthaman, R., Vidyalakshmi, R., Murugesh, S., and Singaravadivel, K, 2009, Optimization of various culture media for tannase production in submerged fermentation by Aspergillus flavus, Adv. Biol. Res., 3, 34–39.

[7] Mondal, K. C., Banerjee, D., Banerjee, R., and Pati, B, 2001, Production and characterization of tannase from Bacillus cereus KBR9, J. Gen. Appl. Microbiol., 47,263–267.

[8] Rodríguez, H., De las Rivas, B., Gómez-Cordovés, C., and Munoz, R., 2008, Degradation of tannic acid by cell-free extracts of Lactobacillus plantarum, Food Chem., 107, 664–670.

[9] Milva, P., Lucia, R. L., Roberto, A., Alessandro, E., Guido, P., Monia, R., Arianna, L., Antonio, F., Simone, G., and Silvano, E. F., 2010, Tannic acid degradation by bacterial strains Serratia spp. and Pantoea sp. isolated from olive mill waste mixtures, Int.Biodeter. Biodegr., 64, 73–80.

[10] Lekha P.K. and Lonsane B.K., 1997, Production and application of tannin acyl hydrolase. State of the art, Advances in Applied Microbiology., 44, 215–260.

[11] Lokeswari.N., 2010, Microbial production of Gallicacid by a mutant strain of Aspergillus oryzae using Cashew husk, Pharmacopore., 1, 112-122.

[12] K.C.Mondal, D. Banerjee, M.Jana and B.R. Pati , 2001, Colorimetric assay method for determination of tannin Acyl hydrolase (EC. 3.1.1.20) activity, Analytical Biochemistry, 295, 168–171.

[13] Sabu A, Pandey A, Jaafar Daud M. and Szakacs G., 2005, Tamarind seed powder and palm kernel cake: two novel agro residues for the production of tannase under solid state fermentation by Aspergillus niger ATCC 16620. Biores. Technol., 96, 1223-1228.

[14] Simeon Chukwuemeka Enemuor and Fredrick John Chidi Odibo, 2010, Partial purification and characterization of Aspergillus tamari IMI388810 (B) tannin acyl hydrolase, Archives of Applied Science Research, 2 (6): 290-299

[15] Deepanjali Lal and Joy Joseph Gardner, 2012, Production, characterization and purification of tannase from Aspergillus niger,European Journal of Experimental Biology, 2 (5):1430-1438

[16] Mohan Kuppusamy, Viruthagiri Thangavelu and Arunkumar Chockalingam., 2012, optimization of submerged fermentative production of Tannase by Aspergillus flavus International Journal of ChemTech Research, 4 (4), 1461-1467

[17] Rakesh Kumar, Jitender Sharma and Randhir Singh, 2007, Production of tannase from Aspergillus ruber under solid-state fermentation using jamun (Syzygium cumini) leaves,Microbiol research ,162, 384-390

[18] Suresh Baladhandayutham and Viruthagiri Thangavelu, 2011, Optimization and Kinetics of Solid-State Fermentative Production of Pectinase by Aspergillus awamori, International Journal of ChemTech Research, 3 (4),1758-1764.

[19] P.K.Lekha and B.K.Lonsane, 1994, Comparative Titres, Location and Properties of Tannin Acyl Hydrolase produced by Aspergillus niger PKL104 in Solid-state, Liquid surface and Submerged fermentations, Process Biochemistry, 29, 497-503.

[20] D.Banerjee, K.C.Mondal and B.R.Pati, 2007, Tannase production by Aspergillus aculeatus DBF9 through Solid state fermentation, Acta Microbiologica et Immunologica Hungarica, 54 (2):159-166.