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Volume 1, Issue 2

International Journal of Science and Engineering Applications (IJSEA)
Volume 1, Issue 2 - December 2012

Production of Tannase using Aspergillus niger by Submerged Fermentation

Mohan Kuppusamy, Viruthagiri Thangavelu, Suresh Baladhandayutham




A study on optimization of submerged fermentative production of tannase by Aspergillus niger was carried out. Aspergillus niger (MTCC 3557) was used for maximum production of Tannase enzyme and the factors affecting the production were optimized. The optimum conditions for maximum tannase enzyme production in the Submerged Fermentation (SmF) using Aspergillus niger was obtained with an initial tannic acid concentration of 3% (w/v) at 35°C, Initial pH of 5.5 and a fermentation period of 96h.The substrates Betalnut husk and Plantain flower gave a maximum tannase production of 19.3 U/ml and 17.89 U/ml respectively.

Keywords: Submerged fermentation, Tannase, Agro residue, Aspergillus niger. optimization.


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