IJSEA Archive (Volume 4, Issue 4)
International Journal of Science and Engineering Applications (IJSEA) (Volume 4, Issue 4 July-August 2015)
Fatty Acid Pattern and Alkaloids of Echium Rauwolfii
Abdelaziz M. Dawidar, Abdul-Sattar H. A Ghani, Maha M. Alshamy, Eman H. Tawfik, Mamdouh Abdel-Mogib
Keywords: fatty acids, pyrrolizidine alkaloids, Echium rauwolfii, Boraginaceae
The GC/MS analysis of hexane extract revealed the presence of palmitic acid as
saturated fatty acid (1.05%), versus oleic acid (2.18%), linoleic acid (1.13%),
cis-8,11,14-eicosatrienoic acid (2.12%) as unsaturated fatty acids. On the other
hand, CH2Cl2 extract contained palmitic acid methyl ester (3.55%), and methyl
isostearate (1.17%) as saturated fatty acids, versus linoleic acid methyl ester
(3.57%) and linolenic acid methyl ester (10.01%) as unsaturated fatty acids. The
GC/MS analysis of the alkaloid-rich fraction indicated the presence of the
pyrazolidine alkaloids petranine (2.97%), 7-angeloyl-9-(2-methylbutyryl)
retronecine (4.22%), 7-angeloylretronecine (0.59%) and 9-angeloylretronecine
(0.47%).
The butanol extract showed the heights DPPH radical scavenging activity (IC50 =
14.3 µg),. while ethyl acetate extract was very weak in activity (IC50 = 432.3
µg) and no activity with hexane and methylene chloride extract.
The antimicrobial potentials of E. rauwolfii extracts were examined. The
inhibition of the fungi species by ethyl acetate extract exert was comparable to
Amphotericin B. The inhibition zone of the butanol extract against Streptococcus
pneumonia was comparable to Ampicillin, against Pseudomonas aeruginosa was
comparable to Gentamicin and Escherichia coli was comparable to Gentamicin.
The cytotoxicity against HePG-2 of ethyl acetate extract and butanol extract
were “very strong”, and that of hexane extract and methylene chloride extract
were “moderate”, against MCF-7 of ethyl acetate extract and butanol extract
were “strong”, that of methylene chloride extract was “moderate”, and that of
hexane extract was “weak” and against HCT-116 of butanol extract was “very
strong”, of ethyl acetate extract was“strong”, of methylene chloride extract and
hexane extract were “moderate”.
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@article{Abdelazizl04041009,
title = "Fatty Acid Pattern and Alkaloids of Echium Rauwolfii ",
journal = "International Journal of Science and Engineering Applications (IJSEA)",
volume = "4",
number = "4",
pages = "208 - 213",
year = "2015",
author = " Abdelaziz M. Dawidar, Abdul-Sattar H. A Ghani, Maha M. Alshamy, Eman H. Tawfik, Mamdouh Abdel-Mogib ",
}