Based on the actual situation of the "C Language Programming" course in IT majors, the characteristics of this course are analyzed, and a research and practice on C language teaching reform based on PI ability cultivation is proposed. Adopting a theme project oriented theoretical teaching method, constructing a "three-level" and "three combination" practical teaching system, implementing diversified evaluation and assessment methods, and improving students' practical and innovative abilities. The experimental teaching of C language is an important part of the C language curriculum. Based on the characteristics of cultivating applied talents in independent colleges, and in response to the problems encountered in experimental teaching, a plan is proposed to optimize the overall content and system of C language experimental teaching, further improving the teaching effectiveness of C language experiments.