Effect of Rhodamine B dye on the growth and properties of L-Alanine Thiourea Bulk single crystals has been reported. The cell parameters and crystallinity of pure and dye admixtured LATU crystals were confirmed by single crystal, powder X-ray diffraction and high resolution X-ray diffraction analyses. The functional groups present in the crystals were confirmed by FTIR analysis. The UV-vis-NIR transmission studies show the optical transparency in the entire visible region of Rhodamine B dye admixtured LATU crystal. The laser damage threshold value significantly enhanced for dye admixtured crystal in comparison with pure LATU crystal. The crystals were further subjected to other important characterizations such as dielectric measurement, micro hardness, thermal and NLO studies. The relative SHG efficiency of Rhodamine B dye admixtured LATU crystal was found to be 1.46 times higher than that of pure LATU crystal.