The study of susceptibility assessment of microbial flora from ready-to-drink herbal medicine was aimed to isolate the identify bacteria associated with ready-to-drink herbal products marketed within Kaduna metropolis. The susceptibility of the isolates to commonly-used antibiotics was also determined. The assessment of the contamination of the herbal products was carried out using standard procedures (total aerobic plate count) while the isolation, identification and antibiogram of selected bacterial pathogens was done using the Kirby-bauer method. The microbial analysis was carried out on twenty one (21) ready-to-drink herbal products with total mean bacterial count ranging from 3.5x107 to 4.14x107cfu/ml. The result also revealed that the sample had 40 bacterial isolates. The herbal drinks had a total mean aerobic bacterial plate count greater than 107cfu/ml. Of all, 32.5% of the herbal products were contaminated with Escherichia coli, 17.5% Bacillus moniliformis, Staphylococcus aureus 22.5%, Pseudomonas aeruginosa 12.5% while 7.5% each was found with Klebsiella spp and Streptobacillus spp respectively. The results of antibiogram on the bacterial isolates revealed that all the bacterial isolates were more susceptible to Ciprofloxacin, Pefloxacin and Gentamycin. The ready-to-drink herbal products marketed in Kaduna metropolis are highly contaminated with potential pathogenic bacterial and its preparation needs strict hygienic practices and sanitation to reduce contamination of the product to make it safe for consumption.